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Molecular Biology

Transcript: Oxygen when burned the carbon stored in them are released into the atmosphere Oxygen comes in and Carbon comes out Molecular Biology Atmosphere Paper/Books When the carbon is contained in an object and won't leave trees are cut down and turned in to... Sugar + Oxygen Cars The carbon along with other gasses trap the heat in the earth's atmosphere. They are called greenhouse gasses. Cellular Respiration Bench Carbon Cycle I can outline the carbon cycle Respiration/Photosynthesis I can explain the relation between respiration and photosynthesis Human Health I can discuss how humans get energy from food I can explain how to keep ourselves healthy Trees fall down and over time turn in to... Carbon comes in and Oxygen comes out Humans are also part of the carbon cycle They inhale oxygen (provided by plants) and exhale carbon plants absorb carbon and release oxygen oxygen taken in by animals along with oxygen they eat food and use both to turn in to energy use for growth and other purposes one of the uses of energy are humans mining for the fossil fuels fossil fuels are considered carbon storage burning the item that is carbon storage releases the carbon back into the atmosphere 6 cups of water required, if not: Dehydration- lack of water Respiration Equation Fossil fuels are also carbon storage until they are mined and burned to power... Carbon Storage Nutrition labels are there for the people who eat the food to know what they are taking in and how much. It is used to make sure you are eating a healthy diet. Plants Absorb the carbon in the atmosphere Through photosynthesis they take in carbon and let out oxygen Money may not grow on trees, but it is from trees Carbon Dioxide Fossil Fuels Photosynthesis Recap Carbon Dioxide + Water + energy

Molecular Biology

Transcript: Molecular biologists look for specific patterns of DNA variation. Takes sequences that are the same and different into consideration. Focus on mutation and recombination. Insects' Cell Division Molecular Distance Maps mtDNA Thank you for your time and attention. Background Study of biology at the molecular level. Used to understand the reactions between various cell systems. 2000's = study of gene structure and function became prominent field. www.news-medical.net/health/What-is-Molecular-Biology.aspx What is Molecular Biology? (2011, February 1). Retrieved September 26, 2014, from http://www.news-medical.net/health/What-is-Molecular-Biology.aspx Hutchinson, F. (2014, September 24). Insect Genomes' Analysis Challenges Universality of Cell Division Proteins. Retrieved September 26, 2014, from http://www.sciencedaily.com/releases/2014/09/140924113748.htm Important Questions that are Addressed by DNA Sequence Alighnment. (n.d.). Retrieved from http://cs.calvin.edu/activities/blasted/intro03.html Lansman, R., Shade, R., Sharpira, J., & Avise, J. (1981, January 1). The Use of Restriction Endonucleases to Measure Mitochondrial DNA Sequence Relatedness in Natural Populations. Retrieved September 26, 2014, from http://faculty.sites.uci.edu/johncavise/files/2011/03/42-mtDNA-techniques.pdf How do we use DNA to determine relatedness between species? DNA Sequence Alignment "Genomes for organisms hold a significant amount of evidence for evolution given that living species share that commonality of basic heredity systems that use DNA and RNA to pass on genes from parent to offspring." Insects' cell division mtDNA DNA sequence alignment Molecular Distance Maps Molecular Biology www.exploredna.co.uk/evolution-dna.html Examples of Evidence

PowerPoint Game Templates

Transcript: Example of a Jeopardy Template By: Laken Feeser and Rachel Chapman When creating without a template... http://www.edtechnetwork.com/powerpoint.html https://www.thebalance.com/free-family-feud-powerpoint-templates-1358184 Example of a Deal or No Deal Template PowerPoint Game Templates There are free templates for games such as jeopardy, wheel of fortune, and cash cab that can be downloaded online. However, some templates may cost more money depending on the complexity of the game. Classroom Games that Make Test Review and Memorization Fun! (n.d.). Retrieved February 17, 2017, from http://people.uncw.edu/ertzbergerj/msgames.htm Fisher, S. (n.d.). Customize a PowerPoint Game for Your Class with These Free Templates. Retrieved February 17, 2017, from https://www.thebalance.com/free-powerpoint-games-for-teachers-1358169 1. Users will begin with a lot of slides all with the same basic graphic design. 2. The, decide and create a series of questions that are to be asked during the game. 3. By hyper linking certain answers to different slides, the game jumps from slide to slide while playing the game. 4. This kind of setup is normally seen as a simple quiz show game. Example of a Wheel of Fortune Template https://www.teacherspayteachers.com/Product/Wheel-of-Riches-PowerPoint-Template-Plays-Just-Like-Wheel-of-Fortune-383606 Games can be made in order to make a fun and easy way to learn. Popular game templates include: Family Feud Millionaire Jeopardy and other quiz shows. http://www.free-power-point-templates.com/deal-powerpoint-template/ Quick video on template "Millionaire" PowerPoint Games Some games are easier to make compared to others If users are unsure whether or not downloading certain templates is safe, you can actually make your own game by just simply using PowerPoint. add logo here References Example of a Family Feud Template PowerPoint Games are a great way to introduce new concepts and ideas You can create a fun, competitive atmosphere with the use of different templates You can change and rearrange information to correlate with the topic or idea being discussed. Great with students, workers, family, etc. For example: With games like Jeopardy and Family Feud, players can pick practically any answers. The person who is running the game will have to have all of the answers in order to determine if players are correct or not. However, with a game like Who Wants to be a Millionaire, the players only have a choice between answers, A, B, C, or D. Therefore, when the player decides their answer, the person running the game clicks it, and the game will tell them whether they are right or wrong.

Molecular Biology

Transcript: B Answer Question #4 Poly A TATA box Promoter region First base 2.Missense mutation Question #4 In silence mutation http://web.expasy.org/translate/ GAC 3.Nonsense mutation http://genetics.bwh.harvard.edu/pph2/index.shtml Molecular Biology 3'-UTR STOP CODON 1.Silent mutation (Aspartic Acid) Question #2 Genomic DNA TAG(UAG) tool ALIGN TGA(UGA) A #1 GGGGGCGGGTTCCAGCGCGGGG http://www.fruitfly.org/seq_tools/promoter.html CCCCAGGGGAGGCCGAGCCCGCCGCCCGGCCCCGCG CAGGCCCCGCCCGGGACTCCCCTGCGGTCCAGGCCGCGCCCCGGGCTCCGCGCCAGCCAATGAGCGCCGCCCGGCCGGGCGTGCCCCCGCGCCCCAAGCATAAACCCTGGCGCGCTCGCGGCCCGGCACTCTTCTATAAACCACAGACTCAGAGAGAACCCACCGGGGGCGGGTTCCAGCGCGGGGATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGTGAGGCTCCCTCCCCTGCTCCGACCCGGGCTCCTCGCCCGCCCGGACCCACAGGCCACCCTCAACCGTCCTGGCCCCGGACCCAAACCCCACCCCTCACTCTGCTTCTCCCCGCAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGGTGAGCGGCGGGCCGGGAGCGATCTGGGTCGAGGGGCGAGATGGCGCCTTCCTCGCAGGGCAGAGGATCACGCGGGTTGCGGGAGGTGTAGCGCAGGCGGCGGCTGCGGGCCTGGGCCCTCGGCCCCACTGACCCTCTTCTCTGCACAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTGGCCATGCTTCTTGCCCCTTGGGCCTCCCCCCAGCCCCTCCTCCCCTTCCTGCACCCGTACCCCCGTGGTCTTTGAATAAAGTCTGAGTGGGCGGCAGCCTGTGTGTGCCTGAGTTTTTTCCCTCAGCAAACGTGCCAGGCATGGGCGTGGACAGCAGCTGGGACACACATGGCTAGAACCTCTCT GGC not End codon promoter sequence Used clustalw tool 5'-UTR ASP -> STOP codon Question #5 Question #2 Sequence B로부터 Translation되는 A.A서열 결정 ASP -> ASP GAC (Aspartic Acid) #1 GAU GAU (Aspartic Acid) end codon 3'-UTR missense mutation이 단백질의 기능적인 변화를 초래할 수 있는지 예측하여라 TAA(UAA) Exon & Intron Start codon Question #3 Used clustalw tool Correct 5'-GTC-3' tRNA but 5'-ATC-3' tRNA (Glycine) Used clustalw tool Take home exam 2 Question #3 5'-UTR GGGGGCGGGTTCCAGCGCGGGGATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTGGCCATGCTTCTTGCCCCTTGGGCCTCCCCCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA B Question #5 7th : GAC (Aspartic Acid) A GAC Genomic DNA 3번문제의 내용을 활용하여 Wobble hypothesis를 설명하라 GCTGGAGCCTCGGTGGCCATGCTTCTTGCCCCTTGGGCCTCCCCCCAG Sequence B상의 coding sequence에서 7번째 codon의 base를 하나씩 변화시켜 다음의 mutation을 유도하라 Question #1 Question #1 DNA sequnce 상에 예상되는 다음 지역을 구하여라 Aspartic Acid(GAC) Other Amino Acid Dep. Biomedical science 20133839 Lee In Chae Thank you Thank you Exon Intron Exon Intron It's is Okay because same A.A

Molecular Biology

Transcript: F MOLECULAR BIOLOGY Group B Proteins involved in homologous recombination in E.Coli E It is a type of genetic recombination where nucleotide sequences are exchanged between two similar or identical molecules of DNA. Basic purpose for recombination is: To repair harmful breaks that occur on both strands of the DNA. It is also used in horizontal gene transfer to exchange genetic material between different strains and species of bacteria and viruses. What is homologous recombination? D Homologous Recombination in E.coli There are two types of pathways in this process: RecBCD pathway: repairing of double strand DNA breaks. RecF pathway: repairing of single-strand gaps. Proteins involved in homologous recombination in E.coli At least 25 gene products are involved in promoting genetic exchange: RecA, RecBCD, RecE, RecF, RecG, RecJ, RecN, RecOR, RecQ, RecT, RuvAB, RuvC, SbcCD, and SSB proteins. DNA polymerase I, DNA gyrase, DNA topoisomerase I, DNA ligase, and DNA helicases. Proteins involved in homologous recombination in E.coli C RecA Weight: 38 Kilodaltons Repairs and maintains the DNA. Is found in every specie. (RAD51 in eukaryotes and RadA in archaea) Comprises of a co-protease in bacterial SOS response. Plays a major role in homologous recombination. Binds strongly and in long clusters to ssDNA to form a nucleoprotein filament. The protein has more than one DNA binding site and can hold a single strand and double strand together. This aids in catalysing DNA synapsis reaction between a DNA double helix and a complementary region of ssDNA which forms a filament known as RecA-ssDNA filament. How RecA works? ... The newly formed filament searches for sequence similarity along the dsDNA. The search process enhances sequence complimentary recognition by stretching of the DNA duplex. Initiates recombining of two DNA double helices and exchange of strands. RecA protein catalyses unidirectional branch migration and produces a heteroduplex DNA that is thousands of base pairs long. RecA also contain an additional site for binding and hydrolysing ATP. B RecBCD What is RecBCD? Is an enzyme that is a prototypical example of a molecular machine: the protein architecture incorporates several autonomous functional domains that interact with each other to produce a complex, sequence-regulated, DNA-processing machine. It is an enzyme that is a prototypical example of a molecular machine: the protein architecture incorporates several autonomous functional domains that interact with each other to produce a complex, sequence-regulated, DNA-processing machine. Functions Homologous recombination Degradation; to protect bacteria from phages and extraneous chromosomal DNA Regulation Cis-acting DNA sequences known as Chi (crossover hotspot instigator). activates recombination promoting fucntions. Interaction causes an attenuation of RecBCD enzymes nulcease activity . Switches the polarity of RecBCD to 5’ strand, changes operation of its motor subunit and instructs the enzyme to begin loading RecA protein on the resultant Chi containing single stranded DNA RecF Protein Involved in DNA metabolism It is required for DNA replication Induces normal SOS response Binds preferentially to single-stranded, linear DNA. It also seems to bind ATP. Molecular Function ATP binding Single stranded DNA biniding Biological process DNA replication DNA synthesis involved in DNA repair Double strand break repair Response to UV SOS response RecR Protein May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO. Molecular function DNA Binding Metal ion binding Biological process DNA recombination DNA synthesis involved in DNA repair Response to UV DNA recombination Involved in DNA repair RecF pathway recombination. RecO A Thank you!

Molecular Biology

Transcript: Dan Marthaler Molecular Biology Central Dogma Central Dogma -The process of turning DNA into RNA and then finally into protein (Morange, "The Central Dogma of Biology"). - "DNA, RNA, and proteins are polymers, so each monomer in them is attached to at most two other units" ("Central Dogma of Molecular Biology", KHAN Academy). -DNA is made up of nuclicic acids. -DNA will then make copies of itself in what is known as "replication" ("Central Dogma of Molecular Biology", KHAN Academy). - DNA is the most basic level of information DNA DNA DNA Picture DNA Picture -DNA is then copied into RNA into what is known as "transcription" ("Central Dogma of Molecular Biology", KHAN Academy). - Similar to DNA, RNA is made up of nucleic acids. RNA RNA RNA Picture RNA Picture - Different from DNA and RNA, proteins are made up of amino acids. - The last step in the process is to turn the information from the RNA into a protein throught a process called "translation"("Central Dogma of Molecular Biology", KHAN Acedemy). Protein Protein Translation Translation Sex Determinations Sex Determinations - There are primary and secondary determinations of sex in humans. - "Primary sex determination is the determination of the gonads" (Gilbert, “Chromosomal Sex Determination in Mammals.”) -Primary determination is formed by chromoses and not often influenced by the enviroment. - Both male and female have an X chromose, a female will have two XX and a male will have one X and one Y, XY. Primary Determination Primary Determination Females are XX Males are XY Females are XX Males are XY - "Secondary sex determination affects the bodily phenotype outside the gonads" (Gilbert, “Chromosomal Sex Determination in Mammals.”) -The female begins develop a cervix, vagina and mammary glands. While the male develops a penis, and prostate glands. - This secondary determination of sex is determined by the hormones. Secondary Determination Secondary Determination Strategy Strategy Competitive Advantages Competitive Advantages Case Study Before After Case Study Q&A Q A &

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